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The Scripps Research Institute Florida is currently seeking a Research Assistant II to join the Genomics Core. The Research Assistant II will report to the Scientific Director of the Genomics Core. The successful candidate will perform the full range of next generation sequencing services and must be able to work efficiently as an individual as part of an energized team with meticulous detail to provide customized high-throughput sequencing solution services to a unique and scientifically diverse research community.
Candidate should have the ability to work independently, with minimal supervision, and in collaboration with Genomics core facility wet-bench and computational personnel, as well as with Faculty investigators and their team members. The selected candidate must have an outstanding understanding of molecular biology, and be competent to conduct a wide variety of standard laboratory procedures related to next-generation sequencing. The ideal candidate will assist in overseeing the core laboratory by receiving and processing samples, communicating effectively with investigators and students at multiple trainee levels, to manage projects effectively, to troubleshoot and modify methods as necessary, and to perform routine equipment maintenance and other basic tasks required to maintain organization and operation of the Core’s lab.
RESPOSIBILITIES AND DUTIES:
- Discuss project goals with client laboratory investigators.
- Receive and organize samples from client laboratories. Construct a wide range of libraries for next generation sequencing analysis. This includes extensive QC analysis of library size distributions, library quantities and purity using multiple complementary methods and approaches including qPCR, Qubit, Agilent Bioanalyzer, and TapeStation analysis.
- Work will involve construction and quality control analysis of targeted DNA or RNA based amplicon libraries, and total RNA, small RNA, mRNA, chemically modified DNA and RNA, ChIP, ATAC-seq, and 10X single-cell sequencing libraries.
- Meticulous record keeping is essential.
- Interface with instrumentation scientists to troubleshoot equipment failures and resolve any issues that arise during run setup, sequencing and data generation.
- Additional duties include maintenance of Core equipment, maintaining consumable inventories and reagent ordering.
- Details of established essential functions for this position will be addressed/discussed during the interview process.
Requires a Bachelor’s degree in a relevant scientific discipline or an equivalent combination of education, training and/or experience from which comparable knowledge, skill and abilities have been attained. Requires 3-5 years experience. Candidates possessing a Master’s degree, 2+ years experience required.
- Florida & Palm Beach County residents are preferred.
- The ideal candidate should have excellent pipetting skills and extensive experience with preparation and quantitation of nucleic acids.
- Experience operating Illumina sequencing platforms, such as NextSeq 500 and MiSeq instruments is a plus. Experience with custom library preparation solutions in addition to those from standard kit-based library methods is highly desirable.
- Experience with computer programming and analysis of next generation sequencing data is highly desirable.
- Specific experience and knowledge in fields including (but not limited to) neuroscience, immunology, microbiology, cancer, cell development, genetics or chemistry is highly desirable.
Commensurate with experience
The above statements describe the level of work performed and expected in general terms. The statements are not intended to list all the responsibilities, duties and/or skills required of employees so classified, and the content herein is subject to change due to the business needs of Scripps Research, with or without notice. Furthermore, nothing in this job description shall be interpreted to be in conflict with or to eliminate or modify in any way the employment-at-will status of Scripps Research staff.
The Scripps Research Institute is an Equal Opportunity Employer. All qualified applicants are encouraged to apply and will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other legally protected characteristic or status.
The safety and well-being of our candidates, our people and their families continues to be a top priority. Until restrictions change, interviews will be conducted virtually.