Full job description: http://www.gordian.bio/s/ExVivoScientist.pdf
Company website: https://www.gordian.bio/
Contact: Akela Kuwahara, firstname.lastname@example.org
Your mission as ex vivo scientist is to ensure that Gordian’s screens result in high-quality data. Specifically, you will be leading our animal tissue collection to next generation sequencing workflow. This involves designing and executing tissue collections from experimental animals, generating and sorting single-cell suspensions of these tissues, and preparing these sorted cells for next generation sequencing. You will be expected to take ownership of this part of our platform, including continuous optimization of protocols, application of new technologies and sequencing modalities, and development of methods required for us to work with new and difficult samples. You will thus be directly responsible for the scientific progress of the company.
Each of the steps in our tissue collection to sequencing pipeline requires careful and timely execution, to capture accurate information from our multi-month experiments. You will be responsible for managing experimental schedules and sample databases for many parallel projects, including coordinating with our heads of gene therapy and bioinformatics, ensuring that each experiment is set up to generate the necessary data. Once data is generated, you will be actively involved in interpretation of this data, using your understanding of biology and the experimental workflows to inform future direction of these analyses.
Starting in your first week at the company, you will be trained in protocols for processing multiple tissues and species, including downstream fluorescent sorting, and next-generation sequencing. By the end of your first three months, you will be generating samples indistinguishable in quality from those of your mentor and will have made one significant improvement to our process. Moving forward, you will have full ownership of ex vivo process development, and be heavily involved in project planning alongside our gene therapy and molecular biology teams.
You’ll need excellent hands for surgical and cell handling techniques. You have experience working with mice and desire to keep doing so. You must be able to execute detailed protocols and remain focused through long workflows, sometimes over a week of packed days. Your role requires exquisite attention to detail, and a mindset of continuous optimization. Experience working with large animals, single cell sequencing, FACS, and/or next-generation sequencing are all pluses.