Generation Bio is innovating genetic medicines to provide durable, redosable treatments for people living with rare and prevalent diseases. The company’s non-viral platform incorporates a novel DNA construct called closed-ended DNA, or ceDNA; a unique cell-targeted lipid nanoparticle delivery system, or ctLNP; and a highly scalable capsid-free manufacturing process that uses its proprietary cell-free rapid enzymatic synthesis, or RES, to produce ceDNA. The platform is designed to enable multi-year durability from a single dose, to deliver large genetic payloads, including multiple genes, to specific tissues, and to allow titration and redosing to adjust or extend expression levels in each patient. RES has the potential to expand Generation Bio’s manufacturing scale to hundreds of millions of doses to support its mission to extend the reach of genetic medicine to more people, living with more diseases, around the world.
We are a thriving, collaborative, creative community of 140 people with diverse talents, beliefs, and experiences headquartered in Cambridge, Massachusetts.
Generation Bio seeks an collaborative, versatile, and highly motivated Senior Associate/Principal Associate Scientist to join our Preclinical Research group. For this lab-based position, the successful candidate will be responsible for developing and implementing assays to support disease biology and advance our non viral gene therapy platform, contributing to our liver disease programs from lead optimization to candidate selection.
- Develop and execute assays to assess protein expression and distribution (e.g. immunofluorescence, Western blot, flow cytometry, qPCR) or biological effects (e.g. localization, enzyme activity, signaling pathway activation/inhibition), from tissue samples, plasma/serum, and cell culture lysates
- Conduct in vitro experiments including cell model characterization, transfection, and end point assays
- Design and troubleshoot experiments, analyze data, and interpret results
- Interface with CROs for specific study areas to coordinate research activities
- Document experiments and summarize studies in written reports and oral presentations
- Bachelor’s or Master’s in a related scientific field (e.g. Biology, Biochemistry, Molecular and Cell Biology)
- Minimum of 3-years relevant laboratory experience
- Excellent lab skills and the ability to work independently to conduct and troubleshoot experiments, as well as analyze, interpret, and present data
- Proficient in protein detection methods including immunostaining, western blot, and enzymatic assays from in vivo (tissue/plasma/serum) or in vitro (cell culture) samples
- Familiarity with cell culture manipulation techniques (e.g., transfections, cell-based assays)
- Experience with cell and tissue staining (IF, IHC, ICC) and imaging /analysis methods a plus (confocal microscopy, flow cytometry)
- Ability to multi-task to support multiple projects and work in a matrixed environment
- Prior experience with gene therapy and/or lead optimization a plus
POSITION: Full-Time, Exempt
EEOC Statement: Generation Bio believes in a diverse environment and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, religion, gender, national origin, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Generation Bio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.