Generation Bio is an innovative genetic medicines company focused on creating a new class of non-viral gene therapy to provide durable, redosable treatments for people living with rare and prevalent diseases. The company’s non-viral platform incorporates a proprietary, high-capacity DNA construct called closed-ended DNA, or ceDNA; a cell-targeted lipid nanoparticle delivery system, or ctLNP; and an established, scalable capsid-free manufacturing process. The platform is designed to enable multi-year durability from a single dose of ceDNA and to allow titration and redosing if needed, and our scalable manufacturing process supports our mission to extend the reach of gene therapy to more people, living with more diseases, in more places around the world. Our vision is to make the ravages of genetic disease as imaginary to the next generation as polio feels to children today.
We are a thriving, collaborative, creative community of 120 people with diverse talents, beliefs, and experiences headquartered in Cambridge, Massachusetts.
Generation Bio seeks an energetic, versatile, and highly motivated Associate Scientist to join our Retinal Biology team, within our Preclinical Research group. The successful candidate will perform assays to support the development of our research platform as well as to help understand disease biology for our retinal disease programs. For this lab-based position, the successful candidate will utilize multiple cell and molecular biology techniques while working with a cross functional team. Familiarity with or a willingness to learn rodent eye dissections is a plus. Research will focus on planning and executing endpoint assays for in vitro studies to advance both the platform and processing ocular tissues to assess distribution and gene expression for our growing pipeline of development candidates.
- Design experiments, analyze data, and interpret results
- Conduct in vitro experiments including cell culture maintenance, transfection, cell model characterization, and end point assays
- Harvest retinal tissue from rodent eyes and perform assays to assess transgene delivery, expression, and biological effects
- Optimize and execute assays to assess distribution, gene expression (e.g. qPCR, ELISA, western blot), and biological effects (e.g. function, localization, enzyme activity, signaling pathway activation/inhibition) in tissue samples and cultured cells
- Summarize studies in written reports and oral presentations
- Minimum Bachelor’s degree in a scientific discipline with 0-2+ years of related experience
- Excellent lab technique and the ability to work independently to design experiments and analyze, interpret, and present data
- Experience extracting DNA/RNA/protein from tissue and/or cells and subsequent evaluation of gene expression by PCR, ELISA, western blot, etc.
- Experience with cell culture manipulation techniques (e.g., transfections, cell-based assays)
- Executes standard experiments with level of rigor necessary to establish credibility of results
- Performs well-defined lab experiments with general supervision and willingly seeks advice, can identify problems, and analyze and interpret results
- Familiarity and/or an interest in learning about retinal biology/ophthalmic disease is a plus
- Experience with (or willingness to learn) dissection methods to isolate retinal tissue from rodent eyes
- Excellent organizational and time management skills to allow effective division of time between lab work, data analysis, and documents results clearly
- Meets challenging goals in a timely manner, with managerial support
- Ability to work in matrixed environment
- Excellent written and verbal communication skills
- Prior experience with gene therapy a plus
POSITION: Full-Time, Exempt
EEOC Statement: Generation Bio believes in a diverse environment and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, gender, religion, national origin, gender identity, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Generation Bio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.