HOW MIGHT YOU DEFY IMAGINATION?
Do more with the knowledge you’re working hard to acquire and the passion you already have. At Amgen, our shared mission—to serve patients—drives all that we do. It is key to our becoming one of the world’s leading biotechnology companies, reaching over 10 million patients worldwide. Through this program, you will acquire the valuable hands-on skills and foundational experience to become the professional you are meant to be in your chosen field.
Postdoctoral Fellow – Biologics (Biologic protein expression, intracellular protein phase separation, and cell phenotype analysis)
This could be your everyday
Let’s do this. Let’s change the world. During this program, you will be provided with an enriching environment to inspire innovation that will contribute to the development of human therapeutics.
The lab of Haruki Hasegawa in Biologics Department at Amgen South San Francisco is seeking a postdoctoral fellow to investigate various intracellular protein phase separation events and cellular phenotypes induced by the overexpression of condensation-prone secretory cargo proteins. The lab’s research interests include the elucidation of cargo-specific biosynthetic requirements for various classes of therapeutically important proteins (PMIDs: 29940176, 28273074, 27086875, 20926308, 21182835), characterization of intracellular protein crystallization and protein phase separation in secretory pathway organelles (PMIDs: 34118277, 30851242, 28944095, 26036200, 24703881, 21464137), understanding the relationships between overexpression-induced cell phenotypes and antibody’s solubility characteristics (PMIDs: 34118277, 28379093, 25484054, 23533417, 22728328), and biotechnological application of intra-ER protein inclusion bodies formation.
The initial research projects of the selected postdoctoral fellow are: (1) characterizing the roles of post-translational modifications and protein-protein interactions in intra-ER protein crystallization event; (2) elucidating the solubility and condensation propensity of a human IgG that induces LLPS protein droplet in the ER lumen during overexpression; (3) exploring the merits of identified 5’-UTRs and various effector genes in enhancing difficult-to-express secretory protein production, to list a few potential projects.
The postdoctoral fellow is expected to develop an independent line of research based on initial projects and to present at scientific conferences and publish in leading journals. The successful candidate will operate in a highly collaborative environment, with opportunities to interact with other postdoctoral fellows, scientists, and drug discovery teams while having access to state-of-the art protein expression technologies and resources at core facilities.
The postdoctoral position is for 3 years with the option to extend for an additional year.
Here’s what it takes to Win
We are all different, yet we all use our unique contributions to serve patients. The dedicated individual we seek is a scientist with these qualifications.
Ph.D. at the time of appointment
• Ph.D. in molecular and cell biology, biochemistry, or related life science field
• Solid publication record and strong oral and written communication skills
• Extensive cell imaging experience in immunofluorescent microscopy (EPI, confocal, high content, etc)
• Proficient skills in flow cytometry, cell sorting, intracellular signaling assays, protein purification, and chromatography
• Cell phenotype morphometric analysis using algorithms and machine learning
• Solid knowledge on secretory protein biosynthesis, folding, assembly, trafficking, and secretion
• Familiarity with various stress response signaling pathways and associated cell-based assay methods
• Research experience in the following areas:
– Intracellular protein phase separation, protein crystallization, or protein condensation
– Cell-based assay development and signaling pathway elucidation
– Recombinant antibody production and expression optimization
– Biochemical and biophysical characterization of purified recombinant antibodies
Some of the vast rewards of working here
As we work to develop treatments that take care of others, so we work to care for our teammates’ professional and personal growth and well-being.
Full support and career-development resources to expand your skills, enhance your expertise, and maximize your potential along your career journey
A diverse and inclusive community of belonging, where teammates are empowered to bring ideas to the table and act
Generous Total Rewards Plan—comprising health, finance and wealth, work/life balance, and career benefits—with compensation and benefits rated above 4 stars (out of 5) on Glassdoor
Equal Opportunity Statement
Amgen is an Equal Opportunity employer and will consider you without regard to your race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, or disability status.
We will ensure that individuals with disabilities are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. Please contact us to request an accommodation.
Amgen will consider for employment qualified applicants with criminal histories in a manner consistent with the San Francisco Fair Chance Ordinance.